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Department of Molecular Biosciences & Bioengineering
University of Hawaii-Manoa
1955 East-West Road, Ag. Science 218
Honolulu, Hawaii 96822
Telephone: (808) 956-8384
FAX: (808) 956-3542
Email:mbbe@ctahr.hawaii.edu

 

Courses

MBBE 621 – Metabolic Engineering

Catalog Description

4 Credits. Principles and methodologies of metabolic engineering. Concepts of metabolic networks. Establishment of metabolic flux analysis and metabolic control analysis. Systems biology framework for integration of mathematical modeling and global measurements at metabolite, protein and transcription levels.

Recommended texts and references

  • Stephanopoulos G, Aristidou AA and Nielsen J. Metabolic engineering: principles and methodologies. San Diego: Academic Press, c1998

  • Lee SY and Papoutsakis ET (eds.), Metabolic Engineering. Marcel Decker, 1999.

  • Voit, EO. Computational Analysis of Biochemical Systems. Cambridge University Press, 2000

Objectives

MBBE 621 offers an introduction to the principles and methodology of metabolic engineering; covers mathematical and experimental techniques for the quantitative description, modeling, control, and design of metabolic pathways; and describes the integration of metabolic modeling and high throughput measurements for systems biology study.

Prerequisites

MATH 311, BIOL 275, MICR 351 or consent.

Schedule

Two 150-minute class sessions/laboratory per week.

Designation

Graduate course.

Topics

  1. Introduction to Metabolism and Metabolic Engineering

  2. Comprehensive Models for Cellular Reactions

  3. Fermentation and Cell Cultures

  4. Enzyme Kinetics and Analysis of Sequences of Reactions

  5. Methods to Alter Gene Expression

  6. Regulation of Metabolic Networks

  7. Metabolic Flux Analysis

  8. Metabolic Control analysis

  9. Functional genomics: microarrays, proteomics and metabolomics

  10. Bioinformatics for reconstruction of metabolic networks

  11. Systems biology frameworks for metabolic engineering.

Contribution to Meeting Professional Component

  • Mathematics and Basic Sciences: Linear algebra is covered and solved using matrix operations. Mathematical models of biological systems are developed.

  • Engineering Topics: Mass balance, mass transport, biochemical stoichiometry and their use in rate equations are covered. Applications of engineering concepts to meet special needs in biology are discussed and practiced.

  • General Education: Students will be expected to demonstrate a general knowledge of the areas covered by the course. This will include the ability to synthesize information to solve real and hypothetical problems in metabolic engineering.

Relation to Program Outcomes

MBBE 621 contributes to the following MBBE Program outcomes:

  • solve problems involving differential equations;

  • solve chemistry problems involving inorganic and organic chemistry; problems involving general- and micro-biology;

  • solve engineering problems involving dynamics, fluid mechanics, and thermodynamics;

  • design a system, component, or process in which biology plays a significant role;

  • design and conduct experiments to gather information for engineering designs;

  • use modern engineering techniques, skills, and tools to define, formulate, and solve engineering problems;

  • the ability to function effectively in multi-disciplinary teams;

  • identify professional and ethical responsibilities when practicing engineering;

  • the ability to communicate effectively in large and small groups

  • understand the impact of engineering solutions on the surrounding context.