Plant Physiologist

Education

Research and Professional experience

1996-present

Affiliate Graduate Faculty, University of Hawaii, Molecular Biosciences and Bioengineering; Tropical Plants and Soil Science

1992-present

Research Plant Physiologist USDA Agricultural Research Service at Hawaii Agriculture Research Center

1991-1992

Postdoctoral Researcher; Supervisor- Dr. David Ow USDA Plant Gene Expression Center, Albany, CA.

Publications Last 4 Years

Ming, R., S. Hou, Y. Feng, Q. Yu, A. Dionne-Laporte, J. Saw, P. Senin, W. Wang, S. Salzberg, X. Wang, E. Lyons, D. Rice, M. Riley, R. Skelton, J. Murray, C. Chen, M. Eustice, E. Tong, H. Albert, R. E. Paull, M.-L. Wang, Y. Zhu, M. Schatz, N. Nagarajan, R. Agbayani, P. Guan, A. Blas, J. Wang, J.-K. Na, T. Michael, E. V. Shakirov, B. Haas, J. Thimmapuram, D. Nelson, H. Tang, J. E. Bowers, J. Suzuki, S. Tripathi, K. Neupane, H. Wei, R. Singh, B. Irikura, N. Jiang, W. Zhang, K. Wall, G. Presting, A. Gschwend, Y. Li, A. Windsor, R. N. Pérez, M. J. Torres, F. A. Feltus, B. Porter, M. Paidi, M.-C. Luo, L. Liu, D. Christopher, P. H. Moore, T. Sugimura, C. dePamphilis, J. Jiang, M. Schuler, T. Mitchell-Olds, D. Shippen, J. Palmer, M. R. Freeling, A. H. Paterson, D. Gonsalves, L. Wang and M. Alam (2007). Genome of the transgenic tropical fruit tree papaya (Carica papaya L.) submitted.

Mangwende, T., Wang, M.-L., Borth, W., Hu, J., Moore, P. H., Mirkov, T. E., and Albert, H. H. 2007. The P0 gene of Sugarcane yellow leaf virus encodes a RNA silencing suppressor with novel activities. In preparation

Wang, M.-L., S. Schenck, and H. Albert 2005 Antibody to a short peptide sequence detected sugarcane yellow leaf virus isolates from several sources. Sugar Cane International 23: 25-27.

Yu, Q., P.H. Moore, H.H. Albert, A.H. Roader, R. Ming 2005 Cloning and characterization of a FLORICAULA/LEAFY ortholog, PFL, in polygamous papaya. Cell Res 15(8): 576-84.

Zhu, Y. J., Agbayani, R., McCafferty, H., Albert, H., Moore., P. H. 2005 Effective selection of transgenic papaya plants with the PMI/Man selection system. Plant Cell Reports, 24: 426–432.

Qiu X, Guan P, Wang M-L, Moore PH, Zhu YJ, Hu J, Borth W, Albert HH. 2005. Sensitive, reproducible quantitative expression analysis of BTH-induced genes in papaya. Physiological and Molecular Plant Pathology 65: 21-30.

Wang M-L, Goldstein C, Su W, Moore PH, Albert HH. 2005. Production of biologically active GM-CSF in sugarcane: a secure biofactory. Transgenic Research 14: 167-78

Zhu YJ, Qiu X, Moore PH, Borth W, Hu J, Ferreira S, Albert HH. 2004. Systemic acquired resistance induced by BTH in papaya. Physiological and Molecular Plant Pathology 63(5): 237-248

Wei H, Wang M-L, Moore PH, Albert HH. (2003). Comparative expression analysis of two sugarcane polyubiquitin promoters and flanking sequences in transgenic plants. J Plant Physiol 160: 1241-1251.

Patents

Albert HH, Wei H, Sugarcane ubi9 gene promoter and methods of use thereof. (2004), US Patent no. 6,706,948

Albert HH, Wei H, Sugarcane ubi9 gene promoter sequence and methods of use thereof. (2004) US Patent no. 6,686,513

Albert HH, Wei H, Promoter of the sugarcane ubi4 gene.(2003), US Patent no. 6,638,766

Abstracts

Guan, P., P. H. Moore and H. H. Albert (2007). RNA-induced gene silencing in papaya. . 19th Ann. CTAHR Student Research Symposium. University of Hawaii, Manoa.

Wang, M.-L., T. Mangwende, W. Borth, T. E. Mirkov, J. Hu, P. H. Moore and H. H. Albert (2007). Constitutive expression of viral suppressors of PTGS in sugarcane Plant and Animal Genome XV Conference, San DIego.

Wang, M.-L., W. Borth, T. Mangwende, T. E. Mirkov, J. Hu, P. H. Moore and H. H. Albert (2006). SCYLV PO : function and potential use to control transgene silencing in sugarcane. Tropical Crop Biotechnology Conference 2006, Cairns, Australia.

Albert, H., T. Mangwende, M.-L. Wang and T. E. Mirkov (2006). Functional Analysis of the P0 Protein of Sugarcane Yellow leaf Virus: a Suppressor of Posttranscriptional Gene Silencing. Plant & Animal Genome XIV, San Diego

Mangwende, T., E. Mirkov, and H. Albert. The P0 Protein of Sugarcane Yellow leaf Virus is a Suppressor of Posttranscriptional Gene Silencing. in Plant & Animal Genome XIII. 2005. San Diego, CA.

Albert, H.H., M.-L. Wang, C. Goldstein, P.G. Lemaux, et al. Does single-copy transgene introduction reduce the frequency of PTGS in sugarcane? in Plant & Animal Genome XII Conference. 2004. San Diego, CA.

Albert, H.H., X. Qiu, M. Wang, and P.H. Moore. Survey of BTH-induced genes in papaya by suppression subtractive hybridization. in Plant Genomics 2003. 2003. University of California, Davis.

Albert, H.H., M.-L. Wang, P.G. Lemaux, and P.H. Moore. The Ac-Ds Transposon System-A Tool for Engineering and Research in Sugarcane. in Plant & Animal Genome XI. 2003. San Diego.

Hsu, H.T., J.A. Aebig, H.H. Albert, and B.L. Zhu. Cucumber Mosaic Virus disease resistance in transgenic plants containing single-chain antibody fragments. in International Congress of Plant Pathology. 2003. Christchurch, New Zealand.

Qiu, X., M. Wang, Y.J. Zhu, P.H. Moore, et al. Global expression profiling of pathogen-defense related genes in papaya. in Plant Biology 2003. 2003. Honolulu.

Qiu, X., Y.J. Zhu, M.-L. Wang, R. Ming, et al. Isolation and characterization of genes involved in papaya (Carica papaya L.) systemic acquired resistance. in International Congress of Plant Pathology. 2003. Christchurch, New Zealand.

Specialized Experience

1. Knowledge of research theories and methods of molecular genetics/genomics (structural and functional), bioinformatics and molecular genetic plant-pathogen interactions.

a. Nucleic acid analysis including northern blot, RNase protection, quantitative RT-PCR, siRNA and miRNA gel blot hybridizations, etc

b. Developed an agrobacterium leaf-infiltration system for gene silencing in papaya as a potential functional genomics tool

c. Identified the P0 protein of Sugarcane yellow leaf virus to be a suppressor of RNA silencing, and characterized the activity and functional domains by deletion analysis in Nicotiana benthamiana

2. Knowledge of eukaryotic genome organization, gene structure and gene regulation.

a. Isolated and characterized two strong constitutive promoters from sugarcane,

b. Characterized stable transgenic rice and sugarcane lines expressing these promoters,

c. US patents issued for these two promoters

d. Produced biologically active human GMCSF in transgenic sugarcane, including field trials

3. Ability to concieve, plan and conduct research using a bionformatics/computational biology

a. Bioinformatic analysis to identify potential miRNA genes in the papaya genome using miRcheck (Jones-Rhoades & Bartel, 2004), a PERL script which uses RNAfold (Vienna RNA package 1.6.3, http://www.tbi.univie.ac.at/~ivo/RNA/) to predict secondary structure of the nt sequence,

b. Bioinformatic analysis to predict tRNA genes in the papaya genome using tRNAscan-SE (Lowe & Eddy, 1997),

4. Skill in presenting information to scientists, producers, and government agencies using conventional, electronic, or multi-media technologies.

Graduate Students (4 total):

Post-docs (7 total):

References

Paul Moore, current supervisor, USDA ARS, paul.moore@ars.usda.gov ,808-486-5411 Stephanie Whalen, director, Hawaii Agriculture Research Center, swhalen@harc-hspa.com , 808-486-5310

Dulal Borthakur, graduate chair Molecular Biosystems & Bioengineering, University of Hawaii, dulal@hawaii.edu , 808-956-6600

Sarah Styan, research scientist, Pioneer Hi-Bred, sarah.styan@pioneer.com, 808-338-8300 x113 Cindy Goldstein, Business and Community Outreach Manager, Pioneer Hi-Bred, cindy.goldstein@pioneer.com , 808-637-0100 x17