Courses
MBBE401/BIOL401 Molecular Biotechnology
(Schedule for spring 2009)
Instructor: Prof. Dulal Borthakur
AgSci 415-F; tel: 956-6600
Text book : Molecular Biotechnology by Bernard R. Glick and Jack J. Pasternak (Third Edition)
Room: BioMed B103 Time: MWF 11:30-12:20
Section I: Introduction and Basic Concepts.
1. Jan 12 Biotechnology: definition, history, commercialization of biology (ch. 1,)
2. Jan 14 Molecular biotechnology biological systems (ch. 2,3)
3. Jan 16 Recombinant DNA technology, restriction enzymes, vectors I (ch. 4)
4. Jan 21 Recombinant DNA technology, restriction enzymes, vectors II (ch. 4)
5. Jan 23 DNA synthesis, sequencing, PCR, oligonucleotides (ch. 5)
6. Jan 26 Manipulation of gene expression in prokaryotes I (ch. 6)
7. Jan 28 Manipulation of gene expression in prokaryotes II (ch. 6)
8. Jan 30 Heterologous protein production in eukaryotes (ch. 7)
Feb 2 EXAM 1 (16%)
Section II: Microbial Synthesis and Commercial Products.
9. Feb 4 Principles of protein engineering I (ch. 8)
10. Feb 6 Principles of protein engineering II (ch. 8)
11. Feb 9 Microbial production of pharmaceuticals (ch. 10)
12. Feb 11 Production of antibodies in E. coli (ch. 10
13. Feb 13 Vector vaccines II (ch. 11)
14. Feb 18 Vaccines, subunit and attenuated I (ch. 11)
15. Feb 20 Using recombinant DNA methods to make commercial products
in microbes (ch. 12)
16. Feb 23 Microbial synthesis of antibiotics, biopolymers. (ch. 12)
Feb 25 Exam 2 (16%)
17. Feb 27 Nitrogen fixation & plant growth-promoting bacteria (ch. 14)
18. March 2 Microbial insecticides. (ch. 15)
Section III: Genetic Engineering of Animals and Plants
19. Mar 4 Genetic engineering plants I, Agrobacterium, biolistics (ch. 17)
20. Mar 6 Genetic engineering plants II, manipulation of gene expression (ch.17)
21 Mar 9 Creating pest -resistant plants. Bt toxin (ch. 18)
22. Mar 11 Altering other properties of plants, disease, stress, flowering (ch.18)
23. Mar 13 Cloning and genetic engineering animals: Transgenic MICE (ch. 19)
24. Mar 16 Screening transgenic mice (ch. 19)
Mar 18 Exam 3 (16%)
SPRING BREAK MARCH 23-27
25. Mar 30 Genetic engineering other animals (ch. 19)
26. Apr 1 Human genome project I: Mapping & cloning of human genes (ch. 20)
27. Apr 3 Human genome project II: Mapping & cloning of human genes (ch. 20)
28. Apr 6 Human gene therapy I (ch. 21)
29. Apr 8 Human gene therapy II (ch. 21)
Section IV: Molecular Diagnostics, Bioremediation, Biomass utilization
30. Apr 10 Antibody-based diagnostics, ELISA (ch.9)
31. Apr 13 DNA-based diagnostic systems, forensics (ch. 9)
32. Apr 15 Molecular diagnosis of disease genes (ch. 9)
April 17 Exam 4 (16%)
33. Apr 20 Microarrays, genomics, bioinformatics
34. Apr 22 Preimplant genetic diagnosis (ch. 9)
35. Apr 24 Bioremediation I: Use of microbes and plants to degrade toxins and unnatural chemicals, xenobiotics (ch. 13)
36. Apr 27 Bioremediation II : Genetic engineering of degradation pathways (ch. 13)
37. Apr 29 Bioutilization of starch and cellulose I (ch. 13)
38. May 1 Bioutilization of starch and cellulose II (ch. 13)
Section VI: Patenting, Regulation, socio-ethical aspects of biotechnology.
39. May 4 Regulation of recombinant DNA, GMOs concerns (ch. 21)
40. May 6 Patenting biotechnology inventions (ch. 22)
May 13 (11:50-1:50) Final exam, Part I (16%); Part II (20%); please arrive at 11:40
Grading: Each midterm exam and the part I of final will contain 100 points, out of which 16 points will come from the attendance of 8 the lectures preceding the exam, and another 16 points will be given for answering the review questions preceding the exam. Therefore, actual points for the exam will be 68. Time for answering the questions will be 50 min. Each of these exams will carry 16% of the final grade. The final part II will contain 100 points from the entire course and will carry 20% of the final grade.
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