Biological Nitrogen Fixation College of Tropical Agriculture and Human Resources (CTAHR) Biological Nitrogen Fixation UH Seal Unversity of Hawaii at Manoa
 
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Quality Control of Inoculants - Resources

A variety of factors combine to create quality in a legume inoculant.  These factors include incorporation of a superior rhizobial strain at a high population density, proper packaging, clear labeling, a formulation that is effective and easy to apply, adequate shelf life of the product, and freedom from excessive contamination.  Since the inoculant consumer cannot estimate quality at time of purchase, the responsibility for providing a high quality product lies with the producer.  Key resource documents on quality control of legume inoculants are provided below.

THE ENUMERATION AND IDENTIFICATION OF RHIZOBIAL BACTERIA IN LEGUME INOCULANT QUALITY CONTROL PROCEDURES
Authors:  Perry E. Olsen, Eve S. Sande and Harold H. Keyser

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Complete WORD Version

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MANUAL OF MARKER METHODS FOR THE STUDY OF MICROBIAL ECOLOGY
Authors:  B.B. Bohlool, E.L. Schmidt,  S.N. May and S.F. Dowdle.  Edited by B.B. Bohlool and E.L. Schmidt.

A practical manual on serological techniques, antibiotic-resistance markers and bacteriophage typing, emphasizing rhizobia.  This manual was written in 1984, and is an excellent guide for use of these non-genetic techniques.  Useful for research and quality control applications. 

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Complete WORD Version

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A SIMPLE AGGLUTINATION TEST FOR THE DETECTION OF BRADYRHIZOBIUM
Author: H.H. Keyser

The quick agglutination test is easy to perform and requires simple equipment and materials.  It is well suited for use with inoculant broth as part of a quality control regime.

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WORD Version

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