Methods in Legume-Rhizobium Technology

1. Collecting Nodules-Isolating Rhizobium
2. Observing the Infection Process
3. Cultural and Nutritional Properties
4. Quantifying Growth of Rhizobium
5. Counting Rhizobium by Plant Infection

6. Developing Antisera
7. Agglutination Reactions-Pure Cultures
8. Agglutination with Nodules
9. Gel Immunodiffision
10. Identifying Nodules with Immunodiffision
11. Develop and Use Fluorescent Antibodies
12. Develop Antibiotic Resistant Rhizobia
13. Antibiotic Resistant Strains-Nodules
14. Identifying Rhizobium using Phages

15. Test Genetic Compatibility with Legumes
16. Screen for N2 Fixation Potential
17. Select Effective Strains in Potted Soil
18. Inoculation Field Trials
19. Soil Fertility & Response to Inoculation

20. Rhizobia Culture in Glass Fermentors
21. Preparing Carrier Materials & Inoculants
22. Preparing Inoculant with Diluted Cultures
23. Testing Survival of Rhizobia on Seed

• APPENDIX 1 Subfamilies of the legumes
• APPENDIX 2 Nodule Preservation
• APPENDIX 3 Media & Staining Solutions
• APPENDIX 4 Reagents
• APPENDIX 5 Buffers
• APPENDIX 6 Barium Sulfate Standards
• APPENDIX 7 Seedling Agar Slants
• APPENDIX 8 Rack for Growth Pouches
• APPENDIX 9 Hosts & Growth Systems
• APPENDIX 10 Surface Sterilization of Seed
• APPENDIX 11 Leonard Jar Preparation
• APPENDIX 12 Injecting and Bleeding Rabbits
• APPENDIX 13 Indirect F.A. Technique
• APPENDIX 14 MPN Calculations
• APPENDIX 15 Acetylene Reduction Technique
• APPENDIX 16 Lime Requirements of Acid Soil
• APPENDIX 17 ANOVA for Rhizobium Strain Experiment
• APPENDIX 18 Coefficient of Variation
• APPENDIX 19 Inoculant Carrier Preparation
• APPENDIX 20 Seed Inoculation Procedure
• APPENDIX 21 Determining Field Capacity of Soil
• APPENDIX 22 Simple Transfer Chamber
• APPENDIX 23 Freeze Drying Cultures
• APPENDIX 24 Source of Rhizobium Strains