University of Hawai‘i at Manoa
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Last updated on Friday, September 11, 2015     Make updates ->

Daniel  Jenkins

Name :

Daniel Jenkins

Title :

Prof

Unit :

Department of Molecular Biosciences & BioEngineering

Address :

1955 East-West Rd. #218
                    Honolulu, HI 96822

Room :

St. John 511

Phone :

(808)956-6069

Fax :

(808)956-3542

E-mail :

danielje@hawaii.edu

Specialties :

Sensors and instrumentation, agricultural diagnostics; molecular testing; remote sensing for natural resource and invasive species management.


Professional Prep/Appointments :


Ph. D. Biological and Agricultural Engineering, University of California, Davis, CA, 2001.
M. Eng. Agricultural and Biological Engineering, Cornell University, Ithaca, NY, 1996.
B.S. Agricultural and Biological Engineering, Cornell University, Ithaca, NY, 1995.

Projects :


USDA-APHISPhysical methods for enhanced surveillance, detection, and control of Coconut Rhinoceros Beetle in Hawaii (proposed). Aug. 1 2016 - July 31, 2017. $149,046
USDA-APHIS: Physical Approaches for Detection and Control of Coconut Rhinoceros Beetle. Aug. 1, 2015 - Jul. 31, 2016. $104,186
Hawaii Invasive Species Committee: Science and Technology Based Management of Incipient Miconia (Miconia calvescens DC) Utilizing Herbicide Ballistic Technology (HBT) (co-PI under PI James Leary from NREM). Sept. 1, 2015 - Aug. 31. 2016. $187,210
USDA-APHIS
Rapid On-site Molecular Diagnostics for Select Agent Ralstonia solanacearum. Oct. 1 2015 - Sept 30, 2016. $57,316.
USDA-SCRICharacterization of Liberibacter populations and development of field detection system for citrus huanglongbing. Mar. 1, 2015 - Feb. 28, 2019. $248,519
Hawaii Invasive Species Committee​: Quantifying outcomes of miconia (Miconia calvescens DC) management projects through advancements in Herbicide Ballistic Technology (HBT) (co-PI under PI James Leary from NREM). Sept. 1, 2015 - Aug. 31, 2016. $66,000
USDA-AFRINew Engineered Approaches for Recovering Disperse Populations of Low-Tolerance Pathogens from Food. Dec, 14, 2014 - Dec 13, 2017. $498,413
NSF-CBERDElectrophysiological characterization of algae: understanding mechanisms of electroflotation for improved dewatering efficiency. Aug. 1. 2012 - Jul. 31, 2014. $99,990
USDA-SBIRMobile gene-based diagnostics for the agricultural and food processing industry (phase I; contract administered through Diagenetix, Inc). 2012. $99,778 (+ $40,000 in state matching funds)



Courses Taught:


BE 120 (Quantitative Biology; Spring 2011, Spring 2012)
BE 150 (Introduction to Biological Engineering; 2005 - 2010)
BE 350/350L (Modeling and Simulation of Dynamic Systems; 2003 - 2007)
BE 420 (Sensors and Instrumentation for Biological Systems; 2004 - present)
BE 606 (Instrumentation and Measurement; starting Spring 2016)
BE/MBBE 625 (Biosensors: Principles and Applications; 2005 - 2008)

Research Interests :


Portable Agricultural Diagnostics: Our laboratory has made significant impacts on developing technologies to facilitate low-cost agricultural diagnostics that can be applied in the field to detect plant disease and otherwise assess plant and animal health. These may also be useful for clinical diagnostics in developing countries operating under many of the resource constraints that generally face the agricultural industry- especially for highly contagious pathogens such as ebola (in humans) or foot and mouth disease (livestock) where transportation of infectious material away from the site of contamination is highly risky. Our lab has developed the first real-time molecular probe ("Assimilating Probes") for use in a truly single step molecular diagnostic reaction conducted under isothermal conditions. This technology has been licensed to the local start-up company Diagenetix Inc., which has also contracted our expertise to develop an extremely cost-effective handheld diagnostic device to conduct testing on-site, and information mangement software to manage and display the results intuitively. Our technologies are currently in use by a spectrum of agricultural industries for assessing disease risk as part of comprehensive management strategies to reduce pesticide use, control disease spread, and prevent introduction of contaminated materials into the food supply. We continue to work on technologies to integrate sample preparation on large statistically relevant agricultural and environmental samples for rapid enrichment and concentration into volumes suitable for rapid molecular assays.

Our lab has also been highly involved in several projects to develop tools to more effectively manage Hawaii's unique natural environment. For example, we have developed instrumentation and real-time map based interfaces to record critical data from manned aerial operations to find and eliminate incipient populations of weeds in extremely remote, rugged, and sensitive habitat in the Hawaiian islands, and are currently working on unmanned and distributed surveillance technologies to autonomously find invasive species to provide alerts of new introductions, or information to guide future control operations.

Selected Recent Publications :


 Peer Reviewed Publications

  • Rodriguez, R., D. M. Jenkins, and J. Leary. 2015. Design and validation of a GPS logger system for recording aerially-deployed herbicide ballistic technology operations. IEEE Sensors 15(4): 2078-2086.
  • Kubota, R. and D. M. Jenkins. 2015. Real-time multiplex applications of Loop Mediated AMPlification by Assimilating Probes. International Journal of Molecular Sciences. 16(3), 4786-4799.
  • Keremane, M. L., C. Ramadugu, E. Rodriguez, R. Kubota, S. Shibata, D. G. Hall, M. L. Roose, D. M. Jenkins, and R. F. Lee. 2015. A rapid field detection system for citrus huanglongbing associated 'Candidatus Liberibacter asiaticus' from the psyllid vector, Diaphorina citri Kuwayama and its implications in disease management. Crop Protection 68:41-48.
  • Jenkins, D. M., J. Jones, and R. Kubota. Evaluation of portable DNA-based technologies for identification of Ralstonia solanacearum race 3 biovar 2 in the field. 2014. Biological Engineering Transactions 7(2):83-96.
  • Yasuhara-Bell, J. H., R. Kubota, D. M. Jenkins, and A. M. Alvarez. 2013. Loop-mediated amplification of the Clavibacter michiganensis subsp. michiganensis micA gene is highly specific. Phytopathology. 103(12):1220-1226.
  • Marrero, G., K. L. Schneider, D. M. Jenkins, and A. M. Alvarez. 2013. Phylogeny and classification of Dickeya based on multilocus sequence analysis. International Journal of Systematic and Evolutionary Microbiology. 63(9):3524-3539.
  • Kubota, R., P. LaBarre, B. H. Weigl, and D. M. Jenkins. 2013. Molecular diagnostics in a teacup: non-instrumented nucleic acid amplification (NINA) for rapid, low cost detection of Salmonella enterica. Chinese Science Bulletin. 58(1):1-7.
  • Jenkins, D. A., P. E. Kendra, N. D. Epsky, W. S. Montgomery, R. R. Heath, D. M. Jenkins, and R. Goenaga. 2012. Antennal responses of West Indian and Caribbean fruit flies (diptera: tephritidae) to ammonium bicarbonate and putrescine. Florida Entomologist. 95(1):28-34.
  • Jenkins, D. M., R. Kubota, J. Dong, Y. Li, and D. Higashiguchi. 2011. Low-cost handheld device for sequence-specific real-time LAMP-based detection of Salmonella enterica. Biosensors and Bioelectronics. 30(1):255-260.
  • Kubota, R., P. LaBarre, J. Singleton, A. Beddoe, B. H. Weigl, A. M. Alvarez, and D. M. Jenkins. 2011. Non-Instrumented Nucleic Acid Amplification (NINA) for rapid detection of Ralstonia solanacearum race 3 biovar 2. Biological Engineering Transactions. 4(2):69-80.
  • Kubota, R., D. M. Jenkins, A. M. Alvarez, and W.-W. Su. 2011. FRET-based assimilating probe for sequence specific real-time monitoring of Loop Mediated isothermal AMPlification. Biological Engineering Transactions. 4(2):81-100.
  • Yang, K., D. M. Jenkins, and W.-W. Su. 2011. Rapid concentration of bacteria using submicron magnetic ion exchangers for improving PCR-based multiplex pathogen detection. Journal of Microbiological Methods. 86(1):69-77.
  • Kubota, R., M. A. Schell, G. D. Peckham, J. Rue, A. M. Alvarez, C. Allen, and D. M. Jenkins.  2011.  In silico genomic subtraction guides development of highly accurate, DNA-based diagnostics for Ralstonia solanacearum race 3 biovar 2 and blood disease bacterium.  Journal of General Plant Pathology. 77(3):182-193.
  • Paret M. L., R. Kubota, D. M. Jenkins, and A. M. Alvarez. 2010. Survival of Ralstonia solanacearum race 4 in drainage water and soil, and detection with immunodiagnostic and DNA-based assays. HortTechnology.  20(3):539-548.
  • Jenkins D. M.,  Song, C., S. Fares, H. Cheng, and D. Barrettino.  2009.  Disposable thermostated electrode for temperature dependent electrochemical measurements.  Sensors and Actuators, B- Chemical. 137(1):222-229.
  • Kutin, R., A. Alvarez, and D. M. Jenkins.  2009.  Detection of Ralstonia solanacearum in natural substrates using phage amplification integrated with real-time PCR assay.  Journal of Microbiological Methods.  76(3):241-246.
  • Teruel, M. J., D. M. Jenkins, and J. I. Reyes de Corcuera.  2009.  Crystallization of b-D-glucose and analysis with a simple glucose biosensorJournal of Chemical Education. 86(8):959-961.
  • Fares, A., M. Safeeq, and D. M. Jenkins. 2009. Adjusting temperature and salinity effects on single capacitance sensors. Pedosphere. 19(5):588-596


Patents

  • Sensor for components of a fluid (US Patent No. 6,287,851, awarded September 2001).
  • Disposable Electrode for Detection of Selected Nucleic Acid Sequences (disclosure, September 2005; utility patent filed September 2006).
  • A simple, rapid, and inexpensive circuit for direct measurement of luminescent lifetimes (disclosure, October 2006).
  • Automatic flush trigger for toilet-trained cats (utility patent filed January 2010)
  • Real-time isothermal sequence specific detection of DNA with non-contact temperature controller (disclosed January 2010 - Utility patent filed June 2011)
  • Real-time microalgae harvesting efficiency monitoring system  (disclosed September 2013).
  • Integrated global position system logging system for electro-pneumatic delivery applications (utility patent filed May 2014).
  • Hardware and mobile software for operation of portable instruments for nucleic acid amplification (design patent filed August 2014).

 



Languages :


English, Spanish