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Projects :

• Potentials of anti‐MSTN proteins to enhance skeletal muscle growth of animals
• Improving the efficiency of pig production by enhancing muscle satellite cell proliferation using myostatin-based biotechnology
• Enhancing the sustainability of grass-fed beef production in Hawaii via carcass and meat quality improvement

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Research activities in my laboratory have been directed towards exploring potential methods of improving animal growth performance and carcass composition based on our current understanding of the molecular mechanisms of action of growth regulation. Recent works are mostly related to myostatin, a potent negative regulator of skeletal muscle growth. 

• Understanding of the mechanisms of action of myostatin in regulating skeletal muscle development and growth.
• Production of recombinant proteins interacting with myostatin, including myostatin prodomain, extracellular domain of activin type IIB receptors and investigation of myostatin suppressing activity of those molecules in vitro and in vivo system. 

In addition, we have been investigated the meat quality characteristics of locally produced, forage-finished beef, as well as exploring innovative ways to improve the marketability of low-value cuts of the forage-finished carcasses.

Recent Publications :

Refereed Papers (last 10 years)

1. Yusuf Mohammed Maaeni, Sang Beum Lee, Dong Hyuck Choi, Yong Soo Kim and Paul Mozdziak. 2018. Cloning of Japanese quail (Coturnix Japonica) follistatin and production of bioactive quail follistatin288 in E. coli. International Journal of Poultry Science. 17(1):8-21.
2. Jackie K. Paquette, Ying Ma, Colleen Fisher, Jinze Li, Sang Beum Lee, James F. Zachary, Yong Soo Kim, Cory Teuscher, and Janis J. Weis. 2017. Genetic control of Lyme arthritis by Borrelia burgdorferi arthritis-associated locus 1 (Bbaa1) is dependent on localized differential production of IFN-β and requires upregulation of myostatin. The Journal of Immunology. 199(10):3525-3534.
3. C.N. Lee, G.K. Fukumoto, M.S. Thorne, M.H. Stevenson, Y.S. Kim, M. Nakahata and R.M. Ogoshi. 2017. Nutrient compositions of sugarcane forages were influenced by season and the time of harvest. Journal of Dairy and Veterinary Sciences 2(1):555579.
4. Sang Beum Lee, Sung Kwon Park and Yong Soo Kim. 2017. Maltose binding protein-fusion enhances the bioactivity of truncated forms of pig myostatin propeptide produced in E. coli. Plos One 12(4): e0174956.
5. Jin-Dan Kang, Seokjoong Kim, Hai-Ying Zhu, LongJin, QingGuo, Xiao-Chen Li, Yu-ChenZhang, Xiao-XuXing, Mei-Fu Xuan, Guang-Lei Zhang, Qi-Rong Luo, Yong Soo Kim, Cheng-Du Cui1, Wen-XueLi1, Zheng-YunCui1, Jin-Soo Kim, and Xi-Jun Yin. 2017. Generation of cloned adult muscular pigs with myostatin gene mutation by genetic engineering. RSC Advances 7:12541-12549.
6. J.D. Berrocoso, R. Kilda, A.K. Singh, Y.S. Kim and R. Jha. 2017. Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken. Poultry Science 96:1573-1580.
7. R.K. Putluru, Y.S. Kim and C.N. Lee. 2016. Differential expression of superoxide mutases (SODs) in bovine corpus luteum during estrous cycle and pregnancy. Pacific Agriculture and Natural Resources. Pacific Agriculture and Natural Resources, Vol. 1:11-20.
8. S.B. Lee, J.H. Kim, D.H. Jin, H.J. Jin and Y.S. Kim. 2016. Myostatin inhibitory region of fish (Paralichthys olivaceus) myostatin-1 propeptide. Comp. Biochem. Physiol. Part B. 194-195:65-70.
9. S.B. Lee, S.K. Park, and Y.S. Kim. 2015. Production of bioactive chicken (Gallus gallus) follistatin-type proteins in E. coli. AMB Express 5:58 doi 10.1186/s13568-015-0142-3
10. S.B. Lee, R. Choi, S.K. Park, and Y.S. Kim. 2014. Production of bioactive chicken follistatin315 in Escherichia coli. Applied Microbiology and Biotechnology 98:10041-10051. doi: 10.1007/s00253-014-6139-z
11. W.Y. Haq, S.K. Kang, S.B. Lee, H.C. Kang, Y.J. Choi, C.N. Lee and Y.S. Kim. 2013. High-level soluble expression of porcine myostatin propeptide in Escherichia coli. Applied Microbiology and Biotechnology 97(19):8517-27. doi:10.1007/s00253-013-5134-0.
12. Sang Beum Lee, Jeong Hwan Kim, Hyung-Joo Jin, Il Shik Shin, and Yong Soo Kim, 2013. High-yield expression and purification of bioactive flatfish (Paralichthys olivaceus) myostatin-1 prodomain in Escherichia coli, American Journal of Biochemistry and Biotechnology 8 (3):195-201 DOI: 10.3844/ajbbsp.2012.195.201
13. Y.S. Kim, B. Fox, K.H. Kim, S.B. Lee, H. J. Jin and C.S. Tamaru. 2013. Immersion bath treatment of tilapia fry with myostatin-1 prodomain does not affect tilapia growth at market size. Aquaculture Research 44:1643-1648. DOI:10.1111/j.1365-2109.2012.03168.x
14. B. Funkenstein, E. Krol, E. Esterin, and Y. S. Kim, 2012. Structural and functional characterizations of activin type 2B receptor (acvr2b) ortholog from the marine fish Sparus aurata: evidence for gene duplication of acvr2b in fish. Journal of Molecular Endocrinology 49(3):175-192.
15. M. Wang, H. Yu, Y.S. Kim, C. A. Bidwell and S. Kuang. 2012. Myostatin facilitates slow and inhibits fast myosin heavy chain expression during myogenic differentiation. Biochemical and Biophysical Research Communications 426(1):83-8.
16. Y.S. Kim, G. Fukumoto, and S. Kim. 2012. Carcass quality and meat tenderness of Hawaii pasture-finished cattle and Hawaii-originated, mainland feedlot-finished cattle. Tropical Animal Health and Production 44:1411-1415.
17. Y.S. Kim, K.H. Kim and C.J. Kim. 2012. Production of bioactive extracellular domain of pig and chicken activin type IIB receptors in Pichia pastoris. Process Biochemistry 47:139-146. doi:10.1016/j.procbio.2011.10.027
18. Z. Li, F. Zeng, A Mitchell, Y.S. Kim, Z. Wu and J. Yang. 2011. Transgenic overexpression of bone morphogenetic protein 11 propeptide in skeleton enhances bone formation. Biochemical and Biophysical Research Communications 416:289-292.
19. S.B. Lee, M.J. Cho, J.H. Kim, Y.S. Kim, H.J. Jin. 2011. Production of bioactive rockfish (Sebastes schlegeli) myostatin-1 prodomain in an Escherichia coli system. Protein Journal 30:52-58.
20. K.H. Kim, Y.S. Kim and J.Z. Yang. 2011. Skeletal muscle hypertrophic effect of clenbuterol is additive to the hypertrophic effect of myostatin suppression. Muscle & Nerve 43:700-707.
21. J. Wang, K.H. Kim, S.K. Kim, Y.S. Kim, Q.X. Li, and S. Jun. 2010. Development of a simple quantification method of Escherichia coli K-12 internalized in baby spinach using Fourier Transform Infrared spectroscopy. International Journal of Food Microbiology 144:147-151.
22. S.B. Lee, Y.S. Kim, M.Y. Oh, I.H  Jeong, K.B. Seong and H.J. Jin. 2010. Improving rainbow trout (Oncorhynchus mykiss) growth by treatment with a fish (Paralichthys olivaceus) myostatin prodomain expressed in soluble forms in E.coli. 2010. Aquaculture 302:270-278.
23. Z.C. Li, B. Zhao, Y.S. Kim and J. Yang. 2010. Administration of a mutated myostatin propeptide to neonatal mice significantly enhances skeletal muscle growth. Molecular Reproduction and Development 77:76-82.
24. N.K. Bobbili, Y.S. Kim, M.A. Dunn, J, Yang and A.Ong. 2008. Effects of maternal immunization against myostatin on postnatal growth and skeletal muscle mass of offspring in mice. Food and Agricultural Immunology 19:93-106.
25. Y.S. Kim, N.K. Bobbili, Y.K. Lee, H.J. Jin and M. Dunn. 2007. Production of a polyclonal antibody against unprocessed chicken myostatin and the effects of in-ovo administration of the antibody on post-hatch broiler growth and muscle mass. Poultry Science 86:1196-1205.
26. S.B. Lee, Y.S. Kim and H.J. Jin. 2007. Characterization and expression pattern of myostatin in the rockfish, Sebastes schlegeli. Journal of Fisheries Science and Technology 10:60-67. 

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