University of Hawai‘i at Manoa
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Last updated on Thursday, June 9, 2016     Make updates ->

Qing X  Li

Name :

Qing X Li

Title :

Professor

Unit :

Department of Molecular Biosciences & BioEngineering

Address :

1955 East-West Rd. #218
                    Honolulu, HI 96822

Room :

Ag Sciences Building 402J

Phone :

(808)956-2011

Fax :

(808)956-3542

E-mail :

qingl@hawaii.edu

Website :

http://web.hawaii.edu/lilab/

Specialties :

Proteomics; Agricultural chemistry and biochemistry; Environmental fate of agrochemicals; Bioremediation; Food safety; Functional foods


Professional Prep/Appointments :


B.S. Agriculture, 1982, Agricultural University of Shandong, China 
Ph.D. Agricultural and Environmental Chemistry, 1990, University of California at Davis
Post-doctoral fellow, 1991-1994, University of California at Berkeley



Projects :


  1. Palm peroxidases. We isolate, clone, engineer and express palm peroxidases for biosynthesis of conductive polymers, biosensing and remediation. We study plant glycoprotein heterogeneity, glycoforms and structure and activity relationship.  
  2. Botanical insecticides. We search for novel bioinsecticides and elucidate their mode of action
  3. Functional foods. We isolate and characterize natural products for Alzheimer's disease and cancer prevention.
  4. Food safety, environmental fate and biomonitoring of agrochemicals. We study pesticides and pollutants in foods and the environment. We develop new immunoassays and other analytical methods. We study gas chromatography separation theory, elution model development and computational simulation.
  5. Microbial stress responses to toxic chemicals and mechanisms of biotransformation.  We isolate novel bacteria, Archaea and fungi for bioremediation of pollutants and biosynthesis of high value products. We also study biocontamination of biofuels and blended fuels.
  6. Proteomics. We study glycoproteomics and phosphoproteomics.


Courses:


Environmental Biochemistry (MBBE412, 3 credits- lecture course)

Advanced Laboratory Techniques (MBBE687, 3 credits- lecture/lab course; Proteomics-focused)



Research Interests :


Our research focuses on agricultural and environmental chemistry and biochemistry in five areas.  Our research addresses problems relevant to agrochemicals and foods for human and environment health.  Proteomics is used as a tool for the studies.

1. Palm peroxidases. We isolate, clone, engineer and express palm peroxidases for biosynthesis of conductive polymers, biosensing and remediation. We study plant glycoprotein heterogeneity, glycoforms and structure and activity relationship. 

2. Botanical insecticides. We search for novel bioinsecticides and elucidate their mode of action. 

3. Functional foods. We isolate and characterize natural products for Alzheimer's disease and cancer prevention.

4. Food safety, environmental fate and biomonitoring of agrochemicals. We study pesticides and pollutants in foods and the environment. We develop new immunoassays and other analytical methods. We study gas chromatography separation theory, elution model development and computational simulation.

5. Microbial stress responses to toxic chemicals and mechanisms of biotransformation.  We isolate novel bacteria, Archaea and fungi for bioremediation of pollutants and biosynthesis of high value products. We also study biocontamination of biofuels and blended fuels.



Publications :


Please see:

Web: http://web.hawaii.edu/lilab/

Web: http://manoa.hawaii.edu/jabsom/proteomics

Selected Publications (out of 280)

279.  Wu, L.; Duan, X.; Liu, C.; Zhang, G.; Li, Q.X. 2016. Phenomenon of dual- and single-retention behaviors of solutes and its validation by computational simulation in linear programmed temperature gas chromatography. Journal of Separation Science doi 10.1002/jssc.201600236

278.  Liang, Z.; Zhang, B.; Su, W.W.; Williams, P.G.; Li, Q.X. 2016. C-Glycosylflavones alleviate tau phosphorylation and amyloid neurotoxicity through GSK3β inhibition. ACS Chemical Neuroscience  doi: 10.1021/acschemneuro.6b00059

277.  Baker, M.R.; Tabb, D.L.; Ching, T.; Zimmerman, L.J.; Sakharov, I.Y.; Li, Q.X. 2016. Site-specific N-glycosylation characterization of windmill palm tree peroxidase using novel tools for analysis of plant glycopeptide mass spectrometry data. Journal of Proteome Research 15(6): 2026-2038

276.  Hennessee, C.T.; Li Q.X. 2016. Effects of polycyclic aromatic hydrocarbon mixtures on degradation, gene expression, and metabolite production in four Mycobacterium species.  Applied and Environmental Microbiology 82(11):3357-3369.

257.  Chu, S.; Wang, J.; Leong, G.; Woodward, L.A.; Letcher, R.; Li, Q.X. 2015. Perfluoroalkyl sulfonates and carboxylic acids in liver, muscle and adipose tissues of black-footed albatross (Phoebastria nigripes) from Midway Island, North Pacific Ocean. Chemosphere 138:60-66.

245.  Baker, M.R.; Zhao, H.; Sakharov, I.Y.; Li, Q.X. 2014. Amino acid sequence of anionic peroxidase from the windmill palm tree Trachycarpus fortunei. J. Agric. Food Chem. 62(49):11941–11948.

231.  Cho, I.K.; Chang, C.L.; Li, Q.X. 2013. Diet-induced over-expression of flightless-I protein and its relation to flight dysfunction in Mediterranean fruit fly, Ceratitis capitata. PLoS ONE 8(12):e81099.

228.  Seo, J.-S.; Keum, Y.-S.; Li, Q.X.  2013. Metabolomic and proteomic insights into carbaryl catabolism by Burkholderia sp. C3 and degradation of ten N-methylcarbamates. Biodegradation 24:795-811.

226.  Baker, M.R.; Li, Q.X. 2013. Guanidination tryptic peptides without desalting for MALDI MS analysis. Anal. Chem. 85(18):8873-8880.

218Wu, L.; Chen M.X.; Chen, Y.; Li, Q.X. 2013. Determination and evaluation of gas holdup time with the quadratic equation model and comparison with nonlinear equation models for isothermal gas chromatography. J. Chromatogr. A 1297:196-203

202.  Wang, D.; Atkinson, S.; Hoover-Miller A.; Shelver, W.; Li, Q.X.  2012. Organic halogenated contaminants in mother-fetus pairs of harbor seals (Phoca vitulina richardii) from Alaska, 2000-2002. J. Hazardous Materials 223-224:72-78.

197.  Wang, J.; Chen, W.F.; Li, Q.X. 2012. Rapid identification and classification of Mycobacterium spp. using whole-cell protein barcodes and selected markers with MALDI TOF MS in comparison with multigene phylogenetic analysis. Anal. Chim. Acta 716:133-137.

182.  Seo, J.-S.; Keum, Y.-S.; Li, Q.X. 2011. Comparative protein and metabolite profiling revealed metabolic network in response to multiple environmental contaminants in Mycobacterium aromativorans JS19b1T. J. Agric. Food Chem. 59(7):2876-2882.

175Wang, D.; Li, Q.X.  2010.  Application of mass spectrometry in the analysis of polybrominated diphenyl ethers.  Mass Spectrometry Reviews 29 (5):737-775.

153.  Chang, C.L.; Cho, I.K.; Li, Q.X. 2009. Insecticidal activity of basil oil, trans-anethole, estragole, and linalool to adult fruit flies of Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae.  J. Economic Entomology 102(1): 203-209.

152.  Hennessee, C.T.; Seo, J.-S.; Alvarez, A.M.; Li, Q.X. 2009. Isolation and characterization of five new polycyclic aromatic hydrocarbon-degrading species isolated from Hawaiian soils:  Mycobacterium crocinum sp. nov., Mycobacterium pallens sp. nov., Mycobacterium rutilum sp. nov., Mycobacterium rufum sp. nov., and Mycobacterium aromaticivorans sp. nov.  Int. J. Syst. Evol. Microbiol. 59(2):378-387.

122.  Lee, S.-E.; Seo, J.S.; Keum, Y.-S.; Lee, K.-J.; Li, Q.X.  2007.  Fluoranthene metabolism and associated proteins in Mycobacterium sp. JS14.  Proteomics 7(12):2059-2069.

082.  Keum, Y.-S.; Li, Q.X.  2005.  Reductive debromination of polybrominated diphenyl ethers by zero-valent Iron.  Environ. Sci. Technol. 39(7):2280-2286.

081.  Hou, S. et al.  2004.  Genome sequence of the deep-sea γ-Proteobacterium Idiomarina Ioihiensis reveals amino acid fermentation as source of carbon and energy.  Proc. Natl. Acad. Sci. USA 101(52):18036-18041.

075.  Keum, Y.-S.; Li, Q.X.  2004.  Copper dissociation as a mechanism of fungal laccase denaturation by humic acid.  Appl. Microbiol. Biotech. 64: 588-592.

051.  Chiu, Y.-W.; Li, Q.X.; Karu, A.E.  2001.  Selective binding of polychlorinated biphenyl congeners by a monoclonal antibody: analysis by kinetic exclusion fluorescence immunoassay. Anal. Chem. 73(22):5477-5484.

046.  Pellequer, J.L.; Zhao, B.; Kao, H.-I.; Bell, C.W.; Karu, A.E.; Li, K.; Li, Q.X.; Roberts, V.A.  2000.  Stabilization of bound polycyclic aromatic hydrocarbons by a p-cation interaction. J. Mol. Biology 302(3):691-699.

045.  David, M.D.; Campbell, S.; Li, Q.X.  2000.  Pressurized fluid extraction of polar herbicides, and polar herbicides using in-situ derivatization.  Anal. Chem. 72(15):3665-3670.

042.  Thomas, S.D.; Li, Q.X. 2000.  Immunoaffinity chromatography for the analysis of polycyclic aromatic hydrocarbons in corals. Environ. Sci. & Technol. 34(12), 2649-2654.

029.  Guo, F.; Li, Q.X.; Alcantara-Licudine, J.P.  1999.  A simple Na4EDTA-assisted sub/supercritical fluid extraction procedure for quantitative recovery of polar analytes in soil.  Anal. Chem. 71:1309-1315.

027.  Li, K.; Chen, R.; Zhao, B.; Liu, M.; Karu A.E.; Roberts, V.A.; Li, Q.X.  1999.  Monoclonal antibody-based ELISAs for part-per-billion determination of polycyclic aromatic hydrocarbons: effects of haptens and formats on sensitivity and specificity. Anal. Chem. 71(2):302-309.

015.  Li, Q.X.; Casida, J.E.  1994.  Structure-activity studies leading to potent chloride channel blockers: 5e-tert-butyl-2-[4-(substituted-ethynyl)phenyl]-1,3-dithianes.  Bioorg. & Med. Chem. 2(12):1423-1434.

005.  Li, Q.X.; Gee, S.J.; McChesney, M.M.; Hammock, B.D.; Seiber, J.N.  1989.  Comparison of an enzyme-linked immunosorbent assay and a gas chromatographic procedure for the determination of molinate residues.  Anal. Chem. 61:818-823.

 

 



Languages :


English, Chinese