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Projects :

• Engineering a yeast biorefinery utilizing renewable oil feedstock from agricultural wastes for high-value oleochemical production

• Value-added products from renewable feedstock via innovative bioprocessing and metabolic engineering

• Engineering novel autoprocessing proteins to advance basic biomedical discoveries, synthesis of protein therapeutics, and agricultural biotechnology

• Engineering next-generation conditional degrons to enable precise temporal control of metabolic networks

• Whole-cell genetically encoded biosensors for PFAS  
  

• Scaling up automated agroinfiltration of hydroponic plants for value-added biomanufacturing  

• Biomanufacturing using machine-learning enhanced yeast cell factory

Courses:

Research Interests :

• Metabolic engineering for improved conversion of lipid feedstock into useful oleochemicals
• Bioprocess and biomolecular engineering related to production of high-value biomass, recombinant proteins, and biochemicals
• Engineering novel synthetic chimeric proteins for novel applications
• Synthetic biology for engineering metabolic pathways and biosensors

Selected Publications :

Su, W.W., Zhang, B. 2015. Auto-processing domains for polypeptide expression. US patent 8,945,876, issued on Feb. 3, 2015.

Su, W.W. 2010. Cooperative reporter systems, components, and methods for analyte detection. US patent 7,741,128, issued on June 22, 2010.

Su, W.W. 2007. Sensor constructs and detection methods. US patent 7,247,443, issued on July 24, 2007.

Chang, S., Christopher, D., Vine, B., Su, W.W., Bugos, R. 2006. Plasmodium falciparum merozoite surface protein-1 malaria vaccine produced in transgenic plants.  US Patent 7,037,681 issued May 2, 2006.

Su, W.W. 1994. External-loop perfusion air-lift bioreactor. U.S. Patent 5,342,781.

Selected peer-reviewed publications:

Worland, A., Han, Z., Maruwan, J., Wang, Y., Du, Z., Tang, Y., Su, W.W.*, Roell, G. W.* 2024. Elucidation of triacylglycerol catabolism in Yarrowia lipolytica: how cells balance acetyl-CoA and excess reducing equivalents. Metabolic Engineering. 85, 1-13. https://doi.org/10.1016/j.ymben.2024.06.010

Han, Z., Maruwan, J., Tang, Y., Su, W.W.* 2023. Conditional protein degradation in Yarrowia lipolytica using the auxin inducible degron. Front. Bioeng. Biotechnol. 11:1188119. doi: 10.3389/fbioe.2023.1188119.

Su, W.W.*, Zhang, B., Han, Z., Kumar, S., Gupta, M., 2022. Non-viral 2A-like sequences for protein coexpression. Journal of Biotechnology, 358, 1-8.

Li, N., Han, Z., O’Donnell, T.J., Kurasaki, R., Kajihara, L., Williams, P.G., Tang, Y.J., Su, W.W.* 2020. Production and excretion of astaxanthin by engineered Yarrowia lipolytica using plant oil as both the carbon source and the biocompatible extractant. Applied Microbiology & Biotechnology, 104, 6977–6989.

Worland, A.M., Czajka, J.J., Xing, Y., Harper, W.F., Moore, A., Xiao, Z., Han, Z., Wang, Y., Su, W. W., Tang, Y.J., 2020. Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock. Metabolic Engineering Communication, 11, e00130.

Worland, A.M., Czajka, J.J., Li, Y., Wang, Y., Tang, Y.J., Su, W.W.* 2020. Biosynthesis of terpene compounds using the non-model yeast Yarrowia lipolytica: grand challenges and a few perspectives. Current Opinion in Biotechnology, 64, 134-140.

Zhang, B., Han, Z.., Kumar, S., Gupta, M., Su, W.W.* 2019. Intein-ubiquitin chimeric domain for coordinated protein coexpression. Journal of Biotechnology, 304, 38-43.

Han, Z., Su, W.W. 2018. Intein-mediated assembly of tunable scaffoldins for facile synthesis of designer cellulosomes. Applied Microbiology & Biotechnology 102(3), 1331-1342.

Han, Z., Park, A., Su, W.W.* 2018. Valorization of papaya fruit waste through low-cost fractionation and microbial conversion of both juice and seed lipid. RSC Advances, 8, 27963-27972. DOI: 10.1039/C8RA05539D

Cho, H.Y., Lee, T., Yoon, Y., Han, Z., Rabie, H., Lee, K.B., Su, W.W., Choi, J.W. 2018. Magnetic oleosome as a functional lipophilic drug carrier for cancer therapy. ACS Appl. Mater. Interfaces. DOI: 10.1021/acsami.7b19255

Zhang, B., Rapolu, M., Kumar, S., Gupta, M., Liang, Z., Han, Z., Williams, P.G., Su, W.W.* 2017. Coordinated protein co-expression in plants by harnessing the synergy between an intein and a viral 2A peptide. Plant Biotechnology Journal 15, 718-728.

Liang, Z., Zhang, B., Su, W.W., Williams, P.G., Li, Q. X. 2016. C-Glycosylflavones Alleviate Tau Phosphorylation and Amyloid Neurotoxicity through GSK3β Inhibition. ACS Chem. Neurosci., 7 (7), 912–923. 

Zhang, B., Rapolu, M., Liang, Z., Han, Z., Williams, P.G., Su, W.W.*  2015. A dual-intein autoprocessing domain that directs synchronized protein co-expression in both prokaryotes and eukaryotes. Scientific Reports 5, 8541; DOI:10.1038/srep08541.

Han, Z., Madzak, C., Su, W.W.*2013.  Tunable nano-oleosomes derived from engineered Yarrowia lipolytica.  Biotechnol. Bioeng. 110(3), 702-710.  

Han, Z, Zhang, B., Wang, Y.E., Zuo, Y.Y., Su, W.W.* 2012. Self-assembled amyloid-like oligomeric-cohesin scaffoldin for augmented protein display on the Saccharomyces cerevisiae cell surface. Appl. Environ. Microbiol. 78(9), 3249-3255.  

Yang, K., Jenkins, D.M., Su, W.W.* 2011. Rapid concentration of bacteria using submicron magnetic anion exchangers for improving PCR-based multiplex pathogen detection. J. Microbiological Methods 86(1), 69-77.   

Zhang, B., Rapolu, M., Huang, L., Su, W.W.* 2011. Coordinate expression of multiple proteins in plant cells by exploiting endogenous kex2p-like protease activity. Plant Biotechnology Journal 9, 970-981. 

Yang, K. & Su, W.W.* 2011. Facile synthesis of metal-chelating magnetic nanoparticles by exploiting organophosphorous coupling. Anal. Biochem. 408(1), 175-177.  

Su, W.W.* 2010. Bioreactors, Perfusion. In: Flickinger, M.C. (ed.) Encyclopedia of Industrial Biotechnology, pp. 978-993, Wiley, New York. (Invited book chapter contribution).

Vardar-Schara, G., Krab, I.M., Yi, G., Su, W.W.* 2007. A homogeneous fluorometric assay platform based on novel synthetic proteins. Biochem. Biophys. Res. Commun. 361, 102-108.

Peckham, G. D., Bugos, R.C., Su, W.W.* 2006. Purification of GFP fusion proteins from transgenic plant cell cultures. Protein Expr. Purif. 49, 183-189.

Su, W.W.*, Liu, B., Lu, W.B., Xu, N.S., Du, G.C., Tan, J.L. 2005. Observer-based online compensation of inner filter effect in monitoring fluorescence of GFP-expressing plant cell cultures. Biotechnol. Bioeng. 91(2), 213-226.

Su, W.W.* 2005. Fluorescent proteins as tools to aid protein production. Microbial Cell Factories 4, 12.

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