University of Hawai‘i at Manoa
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Last updated on Thursday, September 19, 2019     Make updates ->

Jon-Paul  Bingham

Name :

Jon-Paul Bingham

Title :

Assoc Prof

Unit :

Department of Molecular Biosciences & BioEngineering

Address :

1955 East West Rd. #218
                  Honolulu, HI 96822

Room :

Gilmore Hall 111

Phone :


Fax :


E-mail :

Specialties :

Peptide toxins, analytical chemistry, biochemistry

Professional Prep/Appointments :


    • Associate Professor & Graduate Chair – Dept. of Molecular Bioscience and Bioengineering, University of Hawaii, Honolulu, HI, 2014- present
    • Assistant Professor – Dept. of Molecular Bioscience and Bioengineering, University of Hawaii, Honolulu, HI, 2007-2014
    • Assist Research Professor – Dept. of Biology, Clarkson University, Potsdam, NY 2003-2007
    • Post-doctoral position – Dept. of Pharmacology, Yale School of Medicine, New Haven, CT 2000-2003
    • Post-doctoral position – Mass Spectrometry Facility, Dept of Pharmaceutical Chemistry, University of California, San Francisco, CA, 1998-2000
    • PhD. in Biochemistry  – Center for Drug Design and Development, Dept of Biochemistry, University of Queensland, Brisbane, Australia, 1993 – 1998
    • Thesis Title: Novel Toxins From the Genus Conus – From Taxonomy to toxins Research Area:  Proteomics, drug discovery; solid-phase peptide synthesis and biochemistry of peptide toxins Supervisors: Drs. Paul Alewood and Ross Smith
    • B.Sc. (Hons.)  – School of Science, Griffith University, Brisbane, Australia, 1992
    • B.Sc.  – School of Science, Griffith University, Brisbane, Australia, 1989 – 1991

    Professional Membership:

    • The American Peptide Society (2012– present)
    • The American Chemical Society (2006 – present)
    • Advancing Science, Serving Society (AAAS; 2004 – present)
    • The International Society on Toxinology (2003 – present)
    • The Malacological Society of Australasia (1992 – present)
    • The Federation of Clinical Biochemists (1991– present)

    Professional Awards/Recognition:

    • Excellence in Instruction College Teaching Award, University of Hawaii, Manoa – 2011
    • The Peter V. Garrod Distinguished Graduate Mentoring Award, University of Hawaii, Manoa – 2017

    Scientific Advisor/Editor:

    • HI State Pesticide Advisory Committee 2017 - Present
    • HI State Medical Marijuana Advisory Committee (UH Scientific Representative) 2017 - Present
    • Review Editorial Board of Frontiers in Chemical Biology (Frontiers) 2013 – present
    • Member of the Editorial Board – Journal of Toxins (Hindawi Publishing Corp) 2012 – present
    • Vice President of and Program coordinator to the Hawaiian Malacological Society – 2012 – present
    • President of and Scientific Advisor to the Hawaiian Malacological Society – 2011 – 2012
    • Board Director and Scientific Advisor to the Hawaiian Malacological Society – 2009 – 2010

    Projects :

    Pesticide development

    Terrestrial and aquatic mollusks cause serious crop damage, as well as present serious health concerns to both agricultural workers and produce consumers alike. Present molluscicides are ineffective, non-selective, non-biodegradable, residual forming AND costly (reflected in the rising price of copper salts, which are an active constituent in snail baits). Combined, these represent serious concerns in food safety and security, pest management, environmental impact and tropical human health.

    Hawai’i has not escaped these growing concerns, which have seen mass infestations, crop losses (e.g. Taro, water cress, herbs etc.) and has sadly been directly implicated in a number of human deaths on the Big Island (via Angiostrongylus cantonensis / Rat Lung worm – a snail vector-borne parasitic disease). What we are facing here in Hawai’i is not an isolated issue, but is clearly becoming a concern for health care professionals and agriculturalists in both developing and developed countries.

    Noting: Intestinal helminthes (some which are snail vector-borne) are the most common of all human parasites, and schistosomiasis (solely snail vector-borne) is second only to malaria in the public health impact of all vector-borne tropical diseases. Thus the control of snail populations worldwide is directly interrelated to food production/supply, food safety and to human health.

    The primary goal of my research is to interface agriculture research and medicine. We achieve this via researching the use of phyla-selective peptide toxins to control pests – specifically snails, as well as fueling the discovery of new human biopharmaceuticals using novel biosustainable approaches in aquaculture/animal husbandry to ensure a present and future supply of biologics.

    Using venom extracts from the marine snails from the genus Conus, we characterize novel toxin candidates from captive (dietary manipulated) animals, then integrating both synthetic peptide chemistry and bioengineering strategies to produce novel toxin candidate variants for field-testing (and medical research). These efforts include bioengineering of novel fluorescent receptor probes, the establishment of phyla-selective/ receptor isoform-selective high throughput assays for discovery, designing peptide candidate templates for specific applications (i.e. receptor targets) or biological characteristics (i.e. biodegradability).

    The impact of peptide chemistry in medicine has become clearly evident, yet for agricultural applications or use veterinary science there is a significant lag. This is one area that my research laboratory is moving towards changing.

    Value-Added Products from Agriculture - Brewery Sciences



    My mentoring efforts produce independent, highly qualified and competent peptide chemists.  My continued laboratory presence (having an open desk actually inside the working research laboratory) ensures continued student access, intervention, immediate problem solving, greater output and increased student learning. This unique approach adds a dynamic collaborative dimension to the laboratory – my continued presence increases research student presence and productivity.  I lead by example.

    The productivity of the laboratory (student publications and awards) is well recognized by incoming graduate students who request the availability of research positions. Unfortunately every semester I am at capacity (10 graduate students; 4 undergraduates). I mostly recruit prospective graduate students from the MBBE/BIOL 402 Biochemistry class that I teach each fall.  The present trend is that I can secure future graduate students from the top 1-5% of the class. The limited number of undergraduate research students I accept, coming in at a 300-level, often transition to graduate students with accelerated projects once they graduate.

    Mentoring and laboratory-based teaching is one of my daily highlights and provides me with the drive to push the students and myself to new heights in research.

    Title: CSBR-Ownership Transfer: Curate, Preserve and Transfer Agriculturally and

    Environmentally Significant Microorganisms to a Laboratory Equipped for Preservation and


    Objective: Culturing soil bacteria for preservation and distribution (select agents)

    Role: Co-PI

    Agency: NSF



    Title:‘Opihi Aquaculture Year 5 & 6: Improving Hatchery Technology and Production

    Objective: Aquaculture of opihi, the Hawaiian limpets (Cellanaspp)

    Role: PI

    Agency: CTSA




    Title: Investigation of peptide toxin cyclotides as a novel approach to insecticide development

    Objective: Peptide toxin pesticide bioengineering and development

    Role: PI

    Agency: USDA



    Completed Research Support



    Title: Investigating the application of peptide pesticides: Diversifying Molluscicide targeting Capabilities and Enhancing Bio-delivery.

    Objective: Peptide toxin pesticide bioengineering and development

    Role: PI

    Agency: USDA



    Title: Post-harvest management of slugs and snails potentially carrying Rat Lungworm(Angiostrongylus cantonensis)on Hawaii’s farms and gardens.

    Objective: Peptide toxin pesticide discovery and development

    Role: Co-PI

    Agency: USDA-CDC



    Title: Evaluating the Risk of Diphacinone Rodenticide Pellets to Hawaiian Trigger Fish

    Objective: Toxicology of pesticides

    Role: PI

    Agency: US-FWS/HI DNLR




    Title: From Chemistry to Consumption: Exploiting the unique chemical constituency of capsicum frutescensto development a novel Pacific Island crop.

    Objective: Chemical analysis of chili peppers

    Role: PI

    Agency: DOA, HI




    MBBE/BIOL 402 Biochemistry (Spring) (4 Cr)
    MBBE 691 Fermentation Biochemistry (Spring) (3 Cr)
    MBBE 610 Seminar  - Professional Development (Spring/Fall) (1 Cr)
    MBBE 691 Special Topics - Building a better graduate community (Spring/Fall)(1-3 Cr)

    Previously Taught
    BIOL 402L Biochemistry Laboratory (Spring) (2 Cr)

    Professional Interests :

    Peptide protein engineering
    Peptide biochemistry
    Fermentation biochemistry
    Biologically active molecules and their pharmacology - Plant Metabolites/Peptide toxins
    Venomous organisms
    Ion channel targeting and imaging

    Chromatography and Mass Spectrometry
    Chemical Synthesis

    Publications :

    Mau, A. Bingham, J., Soller, F., and Jha, R. (2018) Maturation, spawning, and larval development in captive yellowfoot limpets (Cellana sandwicensis) Invertebrate Reproduction and Development 62(4):239-247.

    Oeser SG, Bingham JP, Collier AC. (2018) Regulation of Hepatic UGT2B15 by Methylation in Adults of Asian Descent.

    Pharmaceutics. 2018 Jan 7;10(1). pii: E6. doi: 10.3390/pharmaceutics10010006.

    Zhang R-Y., Thapa P., M.J. Espiritu M.J., Menon V., Bingham JP. (2017) From Nature to Creation: Going around in Circles, the Art of Peptide Cyclization. Bioorganic & Medicinal Chemistry, 26 (6): 1135-1150.

    Mau, A., Fox, K., and Bingham, J. (2017) The Reported Occurrence of Hermaphroditism in the Yellowfoot Limpet (Cellana sandwicensis Pease, 1981) Annals of Aquaculture and Research 4(4): 1045.

    Thapa P., Cabalteja C.C., Philips E.E. 3rd, Espiritu M.J., Peigneur S., Mille B.G., Tytgat J., Cummins T.R., Bingham J.P. (2016) t-boc synthesis of huwentoxin-i through native chemical ligation incorporating a trifluoromethanesulfonic acid cleavage strategy. Biopolymers. 106(5):737-45

    Zan A. Halford, Peter Y.C. Yu, Robert K. Likeman, Joshua S. Hawley-Molloy, Craig Thomas and Jon-Paul Bingham (2015). Cone shell envenomation: epidemiology, pharmacology and medical care Diving and Hyperbaric Medicine 45 (3):200-207.

    Luzminda Carlos-Hilario,Richard Shimshock, Cherie Ng, Jon-Paul Bingham, David A. Christopher (2015) Screening Carica papaya native promoters driving stilbene synthase expression in Arabidopsis thaliana for resveratrol glucoside (piceid) synthesis. Plant Biotechnology Reports 10.1007/s11816-015-0367-2.

    Aileen Maldonado · Amber Johnson · Deborah Gochfeld · Marc Slattery · Gary K Ostrander · Jon-Paul Bingham · Daniel Schlenk (2015) Hard Coral (Porites lobata) extracts and homarine on Cytochrome P450 expression in Hawaiian butterflyfishes with different feeding strategies.  Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology 08/2015; DOI:10.1016/j.cbpc.2015.08.00

    Huei-Ting Lin · Jan P. Amend · Douglas E. LaRowe · Jon-Paul Bingham · James P. Cowen (2015) Dissolved amino acids in oceanic basaltic basement fluids  Geochimica et Cosmochimica Acta 05/2015; 164. DOI:10.1016/j.gca.2015.04.044

    Thapa P.  Zhang RY. Menon V.  Bingham J-P. (2014) Native Chemical Ligation: A Boon to Peptide Chemistry. Molecules 19(9):14461. DOI:10.3390/molecules19091446 .

    Espiritu M.J, Collier A.C, Bingham J-P. (2014) A 21st Century Approach to Age Old Problems: the ascension of biologics over the small molecule therapeutics. Drug Discovery Today 19 (8), 1109–1113.

    Cleveland V., Bingham J-P., Kan E. (2014) Heterogeneous Fenton degradation of bisphenol A by carbon nanotube-supported Fe3O4. Separation and Purification Technology Volume 133, 8 September 2014, Pages 388–39

    Thapa P., Espiritu M.J., Cabalteja C.C., Bingham J-P. (2014) Conotoxins and their regulatory considerations. Regulatory Toxicology and Pharmacology, 70 (1) 197-202.

    Negi V.S., Bingham J-P., Li Q.X., Borthakur D. (2014) A Carbon-Nitrogen Lyase from Leucaena leucocephala Catalyzes the First Step of Mimosine Degradation. Plant Physiology, 164 (2) 922-934.

    Yafuso J.T, Negi V.S., Bingham J-P., Borthakur D. (2013) Characterization of O-acetylserine (thiol) lyase from Leucaena leucocephala. The FASEB Journal, 27:580.3

    Yafuso J.T, Negi V.S., Bingham J-P., Borthakur D. (2014) An O-Acetylserine (thiol) Lyase from Leucaena leucocephala Is a Cysteine Synthase But Not a Mimosine Synthase. Applied Biochemistry and Biotechnology 173 (5) 1157-1168.

    Espiritu M.J., Cabalteja C.C., Sugai C.K., Bingham J-P. (2014) Incorporation of post-translational modified amino acids as an approach to increase both chemical and biological diversity of conotoxins and conopeptides. Amino Acids 46 (1)125-151

    Takacs Z., Imredy J.P., Bingham J-P., Zhorov B.S., Moczydlowski E.G. (2014) Interaction of the BKCa Channel Gating Ring with Dendrotoxins. Channels, DOI: 10.4161/19336950.2014.949186

    Thapa P., Espiritu M.J., Cabalteja C.C., Bingham J-P. (2014) The Emergence of Cyclic Peptides: The Potential of Bioengineered Peptide Drugs. International Journal of Peptide Research and Therapeutics, DOI: 10.1007/s10989-014-9421

    Bergeron Z.L, Chun J.B, Baker M.R, Sandall D.W, Peigneur S., Yu P.YC, Thapa P., Milisen J.W, Tytgat J., Livett B.G, Bingham J-P. (2013) Analysis of the milked venom from the mollusk-hunting cone shell Conus textile – Peptides, 49, 145–158.

    Kapono CA, Thapa P., Cabalteja CC, Guendisch D. Collier AC and Bingham J-P. (2013) Conopeptide truncation as a post-translational modification to increase the pharmacological diversity within the milked venom of Conus magus – Toxicon 70, 170–178

    Negi V.S., Bingham J-P., Li Q.X., Borthakur D. (2013) midD-encoded 'rhizomimosinase' from Rhizobium sp. strain TAL1145, catabolizes Lmimosine into 3-hydroxy-4-pyridone, Amino Acids. 2013 Jun;44(6):1537-47

    Devappa R.K., Bingham J-P. and Khanal S.K. (2013) New and modified high performance liquid chromatography method for rapid quantification of phorbol esters in Jatropha curcas seed. Industrial Crops and Products 49:211-219

    Bergeron ZL, Bingham JP (2012) Scorpion Toxins Specific for Potassium (K+) channels: A Historical Overview of Peptide Bioengineering. Toxins 4, 1082-1119

    Bingham JP, Andrews EA, Kiyabu SM*, Cabalteja CC (2012) Drugs from Slugs, Part II – Conopeptide Bioengineering. Chemico-Biological Interactions 200 (2012) 92–113 

    Bingham JP, Baker MR, Chun JB. (2012) Analysis of a cone snail's killer cocktail - The milked venom of Conus geographus. Toxicon. Nov;60(6):1166-70 

    Chun JB, Baker MR, Kim D H, Leroy M, Toribo P, Bingham JP (2012) Cone snail milked venom dynamics – a quantitative study of Conus purpurascens. Toxicon. 60(1):83-94 

    Gilly W.F., Richmond T.A., Duda, Jr. T.F., Elliger C., Lebaric, Schulz J., Bingham JP., Sweedler J.V. (2011) A Diverse Family of Novel Peptide Toxins from an Unusual Cone Snail, Conus californicus.The Journal of Experimental Biology. 214:147-61. 

    Bingham JP., Mitsunaga E., Bergeron Z.L. (2010) Drugs from Slugs – Past, Present and Future Perspectives of omega-Conotoxin Research. Chemico-Biological Interactions 183 pp. 1-18.

    Bingham JP, Chun JB, Ruzicka MR, Li QX, Tan ZY, Kaulin YA, Englebretsen DR, Moczydlowski EG. (2009) Synthesis of an iberiotoxin derivative by chemical ligation: a method for improved yields of cysteine-rich scorpion toxin peptides. Peptides. 30(6):1049-57.

    Townsend, A.*, B. G. Livett, J.-P. Bingham, H.-T. Truong, J. A. Karas, P. O’Donnell, N. A. Williamson A. W. Purcell, D. Scanlon, Mass Spectral Identification of Vc1.1 and Differential Distribution of Conopeptides in the Venom Duct of Conus victoriae. Effect of Post-Translational Modifications and Disulfide Isomerisation on Bioactivity. Int. J. Pept. Res. Ther., (2009)15 (3): 195-203.

    Xiao Y, Bingham JP, Zhu, W, Moczydlowski E, Liang S, Cummins TR. (2008) Tarantula Huwentoxin-IV inhibits neuronal sodium channels by binding to receptor site 4 and trapping the domain II voltage sensor in the closed configuration. J Biol Chem. 3;283(40):27300-13.

    Bingham, J-P., Bian, S. Tan, Z-Y., Takacs Z. and Moczydlowski E. (2006) Synthesis of a Biotin Derivative of Iberiotoxin: Binding Interactions with Streptavidin and the BK Ca2+-activated K+ Channel Expressed in a Human Cell Line. Bioconjugate Chem.; 17(3):689 – 699.

    Krishnan M. N., Bingham, J-P., Lee, S. H., Trombley, P.* and Moczydlowski E. (2005) Functional Role and Affinity of Inorganic Cations in Stabilizing the Tetrameric Structure of the KcsA K+ Channel. J Gen Physiol.;126(3):271 – 83.

    Bingham J-P., Broxton N. M.*, Livett L.G,. Down, J. G., Jones A. and. Moczydlowski E.G. (2005) Optimizing the connectivity in disulfide-rich peptides: conotoxin SII as a case study. Anal. Biochem. 338(1):48 – 61.

    Jakubowski, J.A.*, Keays, D.A.*, Kelley, W.P.*, Sandall, D.W*., Bingham, J-P., Livett, B.G., Gayler, K.R. and Sweedler, J.V., (2004) Determining Sequences and Post-Translational Modifications of Novel Conotoxins in Conus victoriae Using cDNA Sequencing and Mass Spectrometry. Rapid communications in Mass. Spect; 34: 548 – 557.

    Marshall, J.*, Kelley, W.P.*, Rubakhin, S. S., Bingham J-P., Sweedlerand Gilly W.F. (2002) Anatomical Correlates of Venom Production in Conus californicus. The biological Bulletin 203, p 27 – 41 

    Hill J. M.*, Oomen C. J., Miranda L. P.*, Bingham J-P.*, Alewood P. F., and Craik D. J. (1998) Three-Dimensional Solution Structure a of a-Conotoxin MII by NMR Spectroscopy: Effects of Solution Environment on Helicity. Biochem, 37, 15621. 

    Broxton, N.*, Down, J., Loughnan, M., Gehrmann, J.*, Bingham, J-P.*, Miranda, L.*, Alewood, P. and Livett, B.G. (1997) Potent a-conotoxins with selectivity for nicotinic receptor subtypes. Proc. Australian Neurosci. Soc. 8: 139. 

    Jones A., Bingham J-P.*, Gehrmann J.*, Bond T., Loughnan M., Atkins A., Lewis R. J., and Alewood P. F. (1996) Isolation and Characterization of Conopeptides by High-performance Liquid Chromatography Combined with Mass Spectrometry and Tandem Mass Spectrometry. Rapid communications in Mass. Spect,. 10, 138.

    Lewis R. J., Bingham J.*, Jones A., Alewood P. F., and Andrews P. R. (1994) Drugs from the peptide venoms of marine Cone Shells. Australian Biotechnology. 4 298 – 300. 

    Published Book Chapters

    Bingham J.*, Jones, A., Alewood, P. F, and Lewis, R. J. (1996) Conus Venom Peptides (Conopeptides): Inter-Species, Intra-Species and Within Individual Variation Revealed by Ionspray Mass Spectrometry. In: Biochemical Aspects of Marine Pharmacology, pp13 – 27. Ed Lazarovici, P., Spira, M. E. and Zlotkin, E., Alaken Inc., Fort Collins, Colorado, USA.

    Bingham J-P., R.K. Likeman, J.S. Hawley, P.Y.C. Yu*, and Z. A. Halford (2014) Conotoxins. In: Manual of Security Sensitive Microbes and Toxins, Ed. D. Liu; CRC Press ISBN: 1466553960

    Published Editorials

    Duda , T.F. Jr. Bingham, J.P., Livett, B.G. Kohn, A.J., Massilia, G. R., Schultz J.R., Down J., Sandall, D., Sweedler J.V. (2004) How much at risk are cone snails? Science. 2004 Feb 13;303(5660):955.

    Bingham J-P., (2010) It’s about being compliant – Insight into the new regulations that govern Federal training awards and grants CTAHR Research News. July-August. Vol, 6 Iss. 6 (50). 19-23.

    Bingham J-P., Chun J.*, Kim D.H.* and Milisen J.* (2010) Local ‘killer’ slugs provide novel leads for medical science and pesticide development. CTAHR Research News. Jan. Vol, 6 Iss. 1 (45). 3-10.