Biological Nitrogen Fixation College of Tropical Agriculture and Human Resources (CTAHR) Biological Nitrogen Fixation UH Seal Unversity of Hawaii at Manoa
 
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Rhizobia, Root  Infection and Nodule Development
These photographs show rhizobia and bradyrhizobia in the free living state, the infection and nodule development process, developed root nodules and nodule contents.

Rhizobia and bradyrhizobia in the free living state.
Rhizobia and bradyrhizobia in the free living state Rhizobia and bradyrhizobia in the free living state
Cells reacted with specific fluorescent antibody Cells reacted with specific fluorescent antibody
Cells reacted with specific fluorescent antibody
Cultures growing on media
B. japonicum on YEM with congo red Various B. spp on YEM with BTB
Left - B. japonicum on YEM with congo red. Right - Various B. spp on YEM with BTB
Various R. spp growing on YEM Various R. spp growing on YEM
Left - Various R. spp growing on YEM.  Right - R. spp. on YEM with congo red.
Selecting antibiotic resistant rhizobia
Selecting antibiotic resistant rhizobia

  
Infection of roots and nodule development
Rhizobia inside infection thread of root hairs Rhizobia inside infection thread of root hairs
Rhizobia inside infection thread of root hairs
Root hairs of soybean
Root hairs of soybean		 Soybean nodule primordia
Soybean nodule primordia
Nodule primordia on steele
Nodule primordia on steele		 Nodule emerging form cortex
Nodule emerging form cortex
Early nodulation on soybean roots
Early nodulation on soybean roots		 Early nodulation on soybean roots
14C labeled soybean root  
14C labeled soybean root. Dark areas show new infection sites and emerging nodules are intense sinks for recent photosynthate (plant shoot pulsed with 14C labeled CO2,  X-ray film developed after contact with root for 24 h).
Ineffective (top) and effective (bottom) soybean nodules Cowpea nodules ranging from completely ineffective (left) to highly effective (right)

Left - Ineffective (top) and effective (bottom) soybean nodules.  Right  - Cowpea nodules ranging from completely ineffective (left) to highly effective (right)
Arachis hypogea (groundnut/peanut) description
Left - Effect of inoculation (left) on nodule number of cowpea planted in a tropical soil. Right - Effects of inoculation on soybean root nodulation in W. Africa.
Arachis hypogea (groundnut/peanut) D. intortum
Left -Arachis hypogea (groundnut/peanut). Right - D. intortum
Stem nodules on Sesbania rostrata Well nodulated soybean root
Left - Stem nodules on Sesbania rostrata. Right - Well nodulated soybean root
description Root hypertrophy on L. leucocephala grown in growth pouches
Left - Leucaena leucocephala. Right - Root hypertrophy on L. leucocephala grown in growth pouches that resembles root nodules
Uninoculated (l) and inoculated (rt) Cicer. Nodulated root of Leucaena leucocephala in field soil
Left - Uninoculated (l) and inoculated (rt) Cicer.  Right -  Nodulated root of Leucaena leucocephala in field soil
Sophora formosa
Sophora formosa

  
Nodule contents
Photomicrographs of rhizobia/bradyrhizobia bacteroids inside nodules Photomicrographs of rhizobia/bradyrhizobia bacteroids inside nodules Photomicrographs of rhizobia/bradyrhizobia bacteroids inside nodules
Photomicrographs of rhizobia/bradyrhizobia bacteroids inside nodules 
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